全文获取类型
收费全文 | 1709篇 |
免费 | 212篇 |
国内免费 | 148篇 |
专业分类
林业 | 7篇 |
农学 | 38篇 |
基础科学 | 2篇 |
14篇 | |
综合类 | 432篇 |
农作物 | 27篇 |
水产渔业 | 451篇 |
畜牧兽医 | 1036篇 |
园艺 | 15篇 |
植物保护 | 47篇 |
出版年
2024年 | 2篇 |
2023年 | 28篇 |
2022年 | 67篇 |
2021年 | 84篇 |
2020年 | 98篇 |
2019年 | 129篇 |
2018年 | 54篇 |
2017年 | 112篇 |
2016年 | 87篇 |
2015年 | 84篇 |
2014年 | 97篇 |
2013年 | 80篇 |
2012年 | 122篇 |
2011年 | 139篇 |
2010年 | 99篇 |
2009年 | 109篇 |
2008年 | 94篇 |
2007年 | 93篇 |
2006年 | 116篇 |
2005年 | 69篇 |
2004年 | 56篇 |
2003年 | 36篇 |
2002年 | 43篇 |
2001年 | 21篇 |
2000年 | 19篇 |
1999年 | 14篇 |
1998年 | 14篇 |
1997年 | 13篇 |
1996年 | 17篇 |
1995年 | 9篇 |
1994年 | 10篇 |
1993年 | 4篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有2069条查询结果,搜索用时 31 毫秒
11.
Weini Zhang Zichao Liao Fengxiao Hu Xinhua Chen Xiaohong Huang 《Aquaculture Research》2019,50(12):3559-3566
Outer membrane proteins (Omps) of Gram‐negative bacteria have been proven to be efficient subunit vaccines against bacteriosis. In this study, OmpF and OmpK of Aeromonas hydrophila were expressed, and their immune protective effects in European eel (Anguilla anguilla) were evaluated. The genomic DNA of A. hydrophila 322A was used as a template, and two kinds of prokaryotic expression plasmids, pET‐32a‐OmpF and pET‐32a‐OmpK, were constructed. Recombinant OmpF protein (r‐OmpF) and r‐OmpK were purified and were proven to have antigenicity by Western‐blot analysis. r‐OmpF and r‐OmpK were used as immunogens to immunize European eel by intraperitoneal injection. The mRNA expression of 6 immune‐related genes (IgM, IL‐10, IRF3, IRF7, LysG4 and HexB) in the liver tissues of eels at 1 hr, 3 hr, 6 hr, 12 hr, 24 hr, 72 hr and 10 days postimmunization was analysed by real‐time PCR. At 30 dpi, the serum antibody response was measured by ELISA. Fish were attacked at 15 dpi by live 322A to assess the protective immunity of r‐OmpF and r‐OmpK. All the six tested genes responded to r‐OmpF or r‐OmpK vaccination at varying degrees. The serum antibody titre of r‐OmpF‐ and r‐OmpK‐immunized groups was 1:1,600 and 1:3,200 respectively. In addition, r‐OmpF gave 35.5% of the relative immune protection rate to European eels, while r‐OmpK gave 70.0%. By analysing the protective immunity and the regulatory role in the immune‐related gene expression of the two recombinant proteins that were studied, it was found that r‐OmpK was a potential vaccine candidate against A. hydrophila. 相似文献
12.
13.
Chenze Zhao Jinyu Zhu Juntao Hu Xiaojing Dong Longsheng Sun Xiaojun Zhang Shuyan Miao 《Aquaculture Nutrition》2019,25(3):712-720
The present study was conducted to investigate the effects of Bacillus pumilus on the growth performance, innate immunity and digestive enzymes of the giant freshwater prawn, Macrobrachium rosenbergii. Prawns (mean weight: 1.81 ± 0.01 g) were fed different levels of B. pumilus at doses of 0, 1 × 107, 1 × 108 and 1 × 109 CFU/g to produce one control group and three experimental groups (G1, G2 and G3), respectively. After a 60‐day experimental feeding period, the results showed that the final weight, weight gain rate (WGR) and specific growth rate (SGR) were significantly higher in experimental groups than in the control (p < 0.05), whereas these improvements did not differ significantly (p > 0.05) among experimental groups. The improvements in phagocytic activity, respiratory burst (RBs) activity and the activity of catalase (CAT), nitric oxide synthase (NOS) and acid phosphatase (ACP) were significantly higher in G2 and G3 than in the control and G1 (p < 0.05). Further, the activity of CAT, NOS and ACP significantly increased with an increase in the dietary level of B. pumilus (p < 0.05). The activity of phenoloxidase (PO) in G3 was significantly higher than that in the control (p < 0.05). The activity of amylase was significantly higher in the groups G2 and G3 than that in the control group. G3 improved the activity of protease compared with the control (p < 0.05). In conclusion, the results of this study indicated that the potential use of B. pumilusat a dose of 1 × 108 CFU/g feed could improve the growth, immunity and digestive enzymes of M. rosenbergii. 相似文献
14.
15.
Yajun Hu Junzhi Zhang Lanbo He Yi Hu Lei Zhong Zhenyan Dai Dinggang Zhou 《Journal of the World Aquaculture Society》2020,51(1):159-170
A 10-week feeding trial aimed to investigate the effects of dietary vitamin C (l -ascorbic acid) on growth, antioxidant activity, and immunity in ricefield eel, Monopterus albus. Six diets were formulated with different levels of vitamin C supplementation (0.1, 17.5, 34.8, 68.6, 139.7, and 278.5 mg/kg). The results indicated that the weight gain (WG) of the M. albus fed the diets with vitamin C was remarkably higher than that of the M. albus fed the control diet (p < .05), and the quadratic analysis showed an optimal vitamin C level of 80.66 mg/kg. Compared with the control group, serum lysozyme activities, the complement pathway, red blood cell counts, white blood cell counts, immunoglobulin M, and phagocytosis percentage were significantly increased (p < .05) in the treatment groups, and superoxide dismutase activity, serum glucose, and malondialdehyde content were significantly decreased (p < .05) in the treatment groups. The challenge trial with Aeromonas hydrophila showed that the M. albus fed the diets with vitamin C had remarkably reduced cumulative mortality compared with those fed the control diet (p < .05), and mortality in the 68.60 mg/kg diet group was the lowest. Based on WG, the optimal vitamin C supplementation level for M. albus was estimated to be 80.66 mg/kg. 相似文献
16.
Effect of the route of administration on the mucosal and systemic immune responses to Lawsonia intracellularis vaccine in pigs 下载免费PDF全文
In an on‐farm study, 40 weaned piglets aged 3 weeks were vaccinated with Lawsonia intracellularis vaccine orally, IM or IP while a fourth group remained unvaccinated. All vaccinated animals showed increased serum levels of L. intracellularis‐specific IgG antibodies, but significantly elevated concentrations of specific IgG, IgA and cytokines were generated in ileal mucosal secretions from the orally and IP vaccinated pigs when examined at 17 days after vaccination. 相似文献
17.
益生菌是一类对宿主机体有益、安全无害的活微生物,代替抗生素和疫苗广泛用于畜禽细菌性肠炎的防治。益生菌主要通过与病原菌竞争黏附位点、分泌抑菌物质、加固肠道黏膜屏障、调节肠道免疫应答等抑制病原菌增殖,缓解肠道炎症,防治细菌性肠炎的发生。本文对益生菌的作用机制进行了详细阐述,为益生菌的研究和应用提供参考。 相似文献
18.
19.
Synbiotic effect of Bacillus mycoides and organic selenium on immunity and growth of marron,Cherax cainii (Austin, 2002) 下载免费PDF全文
The present feeding trial examined the effect of synbiotic use of Bacillus mycoides and organic selenium (OS) as Sel‐Plex on marron immunity, growth and survival. The marron were cultured in recirculated tanks and fed test diets consisting of a basal diet; basal diet supplemented with B. mycoides (108 CFU g?1 of feed); basal diet supplemented with OS (Sel‐Plex) (0.2 g kg ? 1 of feed) and basal diet supplemented with synbiotic (B. mycoides at 108 CFU g?1 and OS 0.2 g kg ? 1 feed) diet, in triplicate. The effect of the prebiotic OS (Sel‐Plex) on the growth rate of B. mycoides was also studied in vitro. The results suggested that synbiotic use of B. mycoides and OS significantly improved some immune parameters of marron, particularly the glutathione peroxidase, and to some extent total haemocyte counts. However, the synbiotic feed did not synergistically improve marron growth, in fact the use of B. mycoides‐supplemented diet alone demonstrated significantly higher growth in marron compared with the growth of marron fed on other test diets. Supplementation of the basal diet with host origin B. mycoides significantly increased the intestinal bacterial population (3.399 ± 825 CFU g ? 1 of gut) in marron compared with other diets. Organic selenium as Sel‐Plex in Trypticase Soya Broth also confirmed that OS did not increase the amount of growth of B. mycoides and resulted in a lower intestinal bacterial population in the synbiotic diet‐fed marron. In conclusion, synbiotic of OS and B. mycoides may improve a particular immune parameters of marron and to a lesser extent their growth. 相似文献
20.
Evaluation of dietary natural mineral materials as an antibiotic replacer on growth performance,non‐specific immune responses and disease resistance in rainbow trout,Oncorhynchus mykiss 下载免费PDF全文
Seonghun Won Mohammad Moniruzzaman Seunghan Lee Jeongwhui Hong Jung‐Keug Park Sangeun Kim Sungchul C Bai 《Aquaculture Research》2017,48(9):4735-4747
We evaluated the effects of some dietary natural mineral materials as an antibiotic replacer based on growth performance, non‐specific immune responses and disease resistance in juvenile and subadult rainbow trout, Oncorhynchus mykiss. First experiment, juvenile rainbow trout averaging 2.7 ± 0.02 g (mean ± SD) were fed one of the six experimental diets; a basal commercial diet as a control (CON), CON with oxytetracycline (OTC), with yellow loess (YL), with Macsumsuk® (MS), with Song‐Gang® stone (SG) and with barley stone (BS) at 0.4% of each diet. At the end of 8‐week feeding trial, weight gain (WG), specific growth rate (SGR), feed efficiency (FE) and protein efficiency ratio (PER) of fish fed YL diet were significantly higher than those of fish fed CON diet. Non‐specific immune responses such as superoxide dismutase (SOD), myeloperoxidase (MPO), lysozyme (LYS) activity and oxidative radical production of fish fed YL diet were higher than those of fish fed CON diet. At the end of 15 days of challenge test with Aeromonas salmonicida, average cumulative survival rate of fish fed YL diet was significantly higher than that of fish fed BS and CON diets. However, there were no significant differences among fish fed YL, SG and OTC diets. Second experiment, subadult rainbow trout averaging 261.5 ± 3.5 g (mean ± SD) were fed one of the four experimental diets for 22 weeks: CON, and CON with OTC, YL or SG at 0.4% of each diet. At the end of feeding, growth performance of fish fed SG and YL diets was significantly higher than that of fish fed CON diet. Non‐specific immune responses in terms of SOD, MPO, LYS and NBT of fish fed SG and YL diets were significantly higher than those of fish fed CON diet. However, there were no significant differences among the fish fed YL, SG and OTC diets. The results indicate that dietary yellow loess or Song‐gang® stone at 0.4% of diet could replace oxytetracycline in juvenile and subadult rainbow trout. 相似文献